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Whole blood cells from normal controls and psoriasis patients were analyzed by <t>cytometry</t> for the cellular phenotype. Results shown are the percentile ranges based on cell counts (cells/µL) of peripheral CD4 + T cells, CD8 + T cells, B cells, monocytes, eosinophils and neutrophils from normal controls ( n = 16) and psoriasis patients in 70mg QD cohort ( n = 12) at baseline (week 0) and week 12. Plot: bottom line, 10 th %; bottom box, 25 th %; top box, 75 th %; top line, 90 th %. *, p < 0.05; **, p <0.01; statistically significant differences between before (week 0) and after (week 12) 70mg QD apilimod treatment in psoriasis patients.
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Whole blood cells from normal controls and psoriasis patients were analyzed by cytometry for the cellular phenotype. Results shown are the percentile ranges based on cell counts (cells/µL) of peripheral CD4 + T cells, CD8 + T cells, B cells, monocytes, eosinophils and neutrophils from normal controls ( n = 16) and psoriasis patients in 70mg QD cohort ( n = 12) at baseline (week 0) and week 12. Plot: bottom line, 10 th %; bottom box, 25 th %; top box, 75 th %; top line, 90 th %. *, p < 0.05; **, p <0.01; statistically significant differences between before (week 0) and after (week 12) 70mg QD apilimod treatment in psoriasis patients.

Journal: PLoS ONE

Article Title: Apilimod Inhibits the Production of IL-12 and IL-23 and Reduces Dendritic Cell Infiltration in Psoriasis

doi: 10.1371/journal.pone.0035069

Figure Lengend Snippet: Whole blood cells from normal controls and psoriasis patients were analyzed by cytometry for the cellular phenotype. Results shown are the percentile ranges based on cell counts (cells/µL) of peripheral CD4 + T cells, CD8 + T cells, B cells, monocytes, eosinophils and neutrophils from normal controls ( n = 16) and psoriasis patients in 70mg QD cohort ( n = 12) at baseline (week 0) and week 12. Plot: bottom line, 10 th %; bottom box, 25 th %; top box, 75 th %; top line, 90 th %. *, p < 0.05; **, p <0.01; statistically significant differences between before (week 0) and after (week 12) 70mg QD apilimod treatment in psoriasis patients.

Article Snippet: Standard template gates were established using FlowJo™ cytometry analysis software (Tree Star, Inc., Ashland, OR) customized for PPD Biomarker/SurroMed to enable upload of gates to our Oracle database.

Techniques: Cytometry